动物造模 z-bio 2023-02-16 482

　　Objective: To study the changes of integrin αvβ3 gene induced differential expression in bovine uterine epithelial cells cultured in vitro, in order to provide reference for exploring the marker proteins of bovine uterine receptivity.

　　METHODS: RT-PCR was used to analyze the changes in the expression of integrin αvβ3 in bovine endometrial epithelial cells induced by different concentrations of estrogen-progestin and estrogen-progestin synergy.

　　Results: When progesterone 10-7 mg/mL was added alone, the expression of integrin αvβ3 was the highest, and the expressions of αv and β3 in the 10-7 mg/mL group were significantly higher than those in the control group ( P < 0.05). In the estrogen 10-10 mg/mL group, the expression of αv was the highest, while the expression of β3 was the lowest. In the synergistic addition of estrogen and progesterone, the expression of integrin αvβ3 was higher than that in the control group, and the expression of αv was significantly higher than that in the control group. higher than the control group ( P<0.05);β3 p="">0.05)?

　　CONCLUSION: The mRNA expression of integrin αvβ3 in bovine endometrial epithelial cells can be increased by adding progesterone alone or in combination with estrogen and progestogen. The effect of adding estrogen alone is the opposite. Therefore, integrin Can αvβ3 be a potential reference marker gene for uterine receptivity in bovine endometrial epithelium during the "implantation window"?