动物造模,全脑缺血再灌注损伤 z-bio 2023-03-09 611

　　Objective: To investigate the protective effect and mechanism of cysteinyl leukotriene receptor antagonists (prolukast, HAMI 3379) on global cerebral ischemia-reperfusion injury in gerbils.

　　Methods: The model of global cerebral ischemia-reperfusion injury in gerbils was established by ligation of bilateral common carotid arteries for 10 min and reperfusion for 24 h. 20 rats in the group were given intraperitoneal injection 3 days before operation, once a day, once 30 minutes before operation. Neurological symptoms and functions were observed at 24 hours after reperfusion; Nissl staining was used to observe neurons in cortex and hippocampus; Western blotting was used to detect the expression of autophagy-related proteins beclin-1 and LC3 in cortex and hippocampus; electron microscope was used to observe autophagosomes in hippocampus . Results Compared with the model group, the prolukast and HAMI 3379 groups could improve the neurological symptom score, reduce the neurological damage, reduce the damage and loss of neurons in the cortex and hippocampus, and reduce the expression of autophagy-related proteins beclin-1 and LC3 and the hippocampus. the number of autophagosomes in the region.

　　Conclusion: Cysteinyl leukotriene receptor antagonist can reduce global cerebral ischemia-reperfusion injury in gerbils by down-regulating autophagy in cerebral cortex and hippocampus.