动物造模,缺氧缺血性脑病 z-bio 2022-10-10 545

　　Objective: To make a hypoxic-ischemic encephalopathy model in neonatal rats, to study the cerebral protective effect of recombinant human erythropoietin (EPO) and the changes of HIE intestinal flora diversity in neonatal rats, so as to provide evidence for clinical application of EPO in the treatment of neonatal rats. Provide experimental evidence for hypoxic-ischemic encephalopathy in children?

　　Methods: 7-day-old SD rats were selected to make HIE model, and randomly divided into HIE model group, EPO experimental group and control group. The expression changes of nestin were observed by immunohistochemistry. 16s rRNA sequencing method to observe the changes of intestinal flora?

　　Results: There were significant differences in the expression levels of nestin in each group at the same time point (P < 0.05), the control group was the lowest, the EPO experimental group was the highest, the HIE model group followed by the Shannon-Wiener index of the HIE model group index) showed a decreasing trend compared to the control group?

　　Conclusion: Exogenous administration of EPO can promote the growth of nerve cells in the neonatal rat model of HIE, and has a certain protective effect. At the same time, the diversity of intestinal flora in rats has changed?