动物造模,抑郁症 z-bio 2022-05-17 496

　　Objective: Taking SD rats as the research object, to explore the effect of histamine on neurons in the hippocampal C1 area and the behavior of depression rats?

　　Methods: The direct effect of histamine on the firing frequency of hippocampal C1 neurons in vitro was observed by the method of extracellular recording of isolated brain slices. The hypothalamus was electrically stimulated by the method of extracellular recording to observe the endogenous histamine. The effect on the firing frequency of hippocampal neurons? Through the open field test and Morris water maze test, to observe the changes of motor desire and spatial memory ability of depression model rats after microinjection of histamine in the hippocampal C1 area?

　　Results: The extracellular recording data of isolated brain slices showed that histamine exerted a concentration-dependent excitatory effect on hippocampal neurons through H1 receptors. Two-way effect of long-term inhibition? In behavioral experiments, the injection of histamine in the hippocampus C1 area significantly reduced the desire to exercise in the open field experiment, and the spatial memory ability was significantly weakened in the Morris water maze experiment, while the injection of histamine H3 The receptor blocker clobenProPit can significantly improve motor desire and spatial memory in depressed rats?

　　CONCLUSION: At the cellular and overall levels, the analysis from different perspectives in vitro and in vivo shows that histamine has a bidirectional effect on neurons in the hippocampal C1 area that is first excited and then inhibited. Spatial memory is weakened, while the histamine H3 receptor blocker clobenProPit can improve motor desire and spatial memory in depressed rats?