动物造模 z-bio 2022-05-26 281

　　Objective: To study the effect of intraosseous local single injection of low-dose simvastatin on angiogenesis and cardiac function in rats after myocardial infarction.

　　Methods: Wistar rats were randomly divided into sham operation group, myocardial infarction model group and intraosseous simvastatin group (n=12). A rat model of myocardial infarction was established by ligation of the left anterior descending coronary artery. After 24 hours, the experimental group received a single injection of simvastatin 0.5 mg into the left tibia. After 4 weeks, the left ventricular function was evaluated by small animal echocardiography. The myocardial infarction area was calculated by triphenyltetrazolium chloride (TTC) staining. Fluorescence staining was used to detect local angiogenesis.

　　RESULTS: Echocardiography showed that the left ventricular systolic function decreased significantly 4 weeks after myocardial infarction, and intraosseous injection of simvastatin did not significantly improve left ventricular function in rats after myocardial infarction; TTC staining showed that the myocardial No significant reduction in infarct size was found; immunofluorescence staining showed no significant increase in myocardial vascular density in the intraosseous simvastatin group.

　　Conclusion: A single intraosseous injection of low-dose simvastatin (0.5 mg) 24 hours after myocardial infarction in rats does not significantly improve myocardial infarction size, angiogenesis and cardiac function.