动物造模,黑色素瘤 z-bio 2022-05-18 439

　　Objective: To investigate the effect of Mac-1 deletion on melanoma tumor growth.

　　Methods: Mac-1 knockout (Mac-1-/-) mice were expanded to obtain the required number of mice for the experiment. The control group C57BL/6J mice and the experimental group Mac-1-/- mice were set up, and B16-F10 cells were injected subcutaneously, respectively, and the survival rate of the mice was counted, and the tumor volume and weight were measured. Macrophage infiltration.

　　Results: The survival rate of melanoma mice after Mac-1 deletion was significantly different from that of control group C57BL/6J mice (P<0.001), and the tumor volume and weight were significantly different from those of control group C57BL/6J mice (P<0.001). <0.001), the number of proliferating cells in tumor tissue was significantly different from that in the control group (P<0.01), and the infiltration of macrophages in tumor tissue was significantly reduced.

　　Conclusion: Mac-1 gene deletion significantly inhibits melanoma tumor growth.