动物造模,2型糖尿病 z-bio 2022-03-30 607

　　OBJECTIVE: To compare the healing characteristics of ulcer wounds in C57BL/6J-db/db and STZ-induced C57BL/6J mice with type 2 diabetes mellitus commonly used, and to conduct relevant animal experiments for the application of a stable diabetic mouse ulcer model Provide evidence.

　　Methods: A diabetic mouse ulcer model was established, and the dynamic wound healing rate and healing time of the mice were counted on 0, 3, 5, 7, 10, and 14 days. and PCNA) to observe the pathological changes of the wounds; the gene expression of the healing-related factors collagen-Ⅲα and α-SMA were quantitatively determined by fluorescence.

　　Results: Compared with STZ-induced mice, the wound healing time of mutant db/db mice was significantly delayed, from (16.6±0.8) d to (20.2±1.3) d (P<0.001); granulation in db/db group was longer than that in STZ group Slow tissue growth, insufficient length of new epithelium, disordered collagen deposition, and poor wound healing; 7 days, the expression of CD31 and PCNA in the db/db group was significantly lower (P<0.01); 7 and 14 days, the genes in the db/db group were up-regulated The multiple of the amount was significantly lower than that of the STZ group.

　　Conclusion: The wounds of the two types of diabetic mice have delayed healing, but the mutant diabetic db/db mice are more suitable for diabetic ulcer wounds in terms of delayed wound healing and ease of healing compared with the STZ-induced mice. Research.