动物造模 z-bio 2023-03-01 578

　　Objective: To investigate the expression levels and significance of Maspin and IKKα in the sinus mucosa of rats with fungal rhinosinusitis (FRS).

　　Methods: A fungal sinusitis model was established in 40 SD rats. According to the random number table method, they were divided into nasal congestion group, FRS group, immunosuppressant group, and invasive FRS group, 10 rats in each group, and 10 healthy rats were selected. Rats were used as blank control group; the control group was fed normally; the nasal congestion group was only injected with hemostatic cotton in the nasal cavity, and the same volume of 0.9% NaCl injection was administered into the abdominal cavity and the nasal cavity; the FRS group was injected with the same volume of 0.9% NaCl injection into the intraperitoneal cavity and intranasal injection of Aspergillus fumigatus Spore suspension; the immunosuppressive group was given intraperitoneal injection of cyclophosphamide and intranasal injection of the same amount of 0.9% NaCl injection; the invasive FRS group was given intraperitoneal injection of cyclophosphamide and intranasal injection of Aspergillus fumigatus spore suspension to establish an invasive FRS rat model . The expression levels of serum interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) were detected by enzyme-linked immunosorbent assay; the expression levels of Maspin and IKKα in nasal tissue of rats in each group were detected by immunohistochemical staining. The expression levels of Maspin mRNA and IKKα mRNA in nasal tissue of rats in each group were detected by quantitative PCR.

　　Results: There were significant differences in the expression levels of serum IL-6 and TNF-α in each group of rats (P<0.05), among which the serum IL-6 and TNF-α in the FRS group were significantly higher than those in the control group, nasal congestion group, and immunosuppressive agents. group and invasive FRS group [(69.3±10.9) ng/L vs (45.2±7.1) ng/L, (46.4±6.7) ng/L, (21.3±4.5) ng/L, (20.9±4.3) ng/L] L; (30.4±4.8) ng/L vs (14.8±2.7) ng/L, (13.9±1.4) ng/L, (7.9±0.6) ng/L, (7.8±0.4) ng/L] (P < 0.05), the serum IL-6 and TNF-α in the control group were significantly higher than those in the immunosuppressive group and the invasive FRS group (P<0.05). there="" was="" no="" significant="" difference="" in="" expression="" levels="" p="">0.05). Immunohistochemical staining results showed that the expression level of Maspin protein in FRS group and invasive FRS group was significantly lower than that in control group, nasal congestion group and immunosuppressive group, and the expression level of IKKα protein was significantly higher than that in control group, nasal congestion group and immunosuppressive group (P < 0.05); the expression of Maspin protein in the aggressive FRS group was significantly lower than that in the FRS group, and the expression of IKKα protein was significantly higher than that in the FRS group (P < 0.05). The expression levels of Maspin mRNA in the FRS group and the aggressive FRS group were significantly lower than those in the control group, nasal congestion group, and immunosuppressive group, and the expression level of IKKα mRNA was significantly higher than that in the control, nasal congestion, and immunosuppressive groups (P<0.05). The expression of Maspin mRNA in the sexual FRS group was significantly lower than that in the FRS group, and the mRNA expression of IKKα was significantly higher than that in the FRS group (P<0.05).

　　Conclusion: The down-regulation of Maspin expression after activation of IKKα is the main cause of the pathogenesis of FRS, and this process may also be one of the molecular mechanisms of invasive FRS.