动物造模 z-bio 2022-03-30 321

　　OBJECTIVE: To compare the bone-improving effect of calcium chelated collagen polypeptide (CPCC) with that of estrogen, and to lay a foundation for the research and development of safe osteoporosis prevention and treatment drugs.

　　METHODS: Rat bilateral ovaries were removed. Ovariectomy group (OVX), sham operation group (sham), 17β-estradiol (OVX+E2) injection group and CPCC gavage group (OVX+CPCC) were set. After 9 weeks The skeletal indexes and blood biochemical indexes of each group were compared.

　　RESULTS: The femoral bone mineral density of the OVX group was significantly lower than that of the sham group (P<0.01), indicating="" the="" occurrence="" of="" op.="" like="" cpcc="" can="" effectively="" inhibit="" abnormal="" changes="" measured="" indicators="" and="" maintain="" them="" at="" level="" sham="" group="" p="">0.05). However, in terms of inhibiting weight gain, the body weight of the E2 group was significantly lower than that of the sham group at the 8th and 9th week (P<0.01); in terms of preventing the loss of bone mineral and bone organic matter, the Mg and Ca levels of the E2 group were significantly lower than those of the CPCC group. , high-dose group, Cu level was no different from sham group, while CPCC (medium and low dose) was significantly higher than sham group. The levels of Mn, Zn, and hydroxyproline in the E2 group were significantly lower than those in the sham group, while CPCC could be maintained at the level in the sham group. The inhibitory effect on the increase of BGP and StrACP levels in blood was not significantly different between the E2 group and the OVX group, but the CPCC group was significantly lower than the OVX group. There was no significant difference between the E2 group and the OVX group in inhibiting the decrease of blood calcium level, while the CPCC group was significantly higher than the OVX group.

　　Conclusion: Calcium-chelating collagen polypeptide is more effective than estrogen in improving bone quality in ovariectomized rats.