动物造模,巨噬细胞 z-bio 2022-11-08 545

　　Objective: To investigate the role of macrophage NF-κB activating factor 1 in inflammatory bowel disease.

　　Methods: The mice whose macrophage Act1 expression was inhibited by targeted inhibition (anti-Act1) were used as the research objects, and the ulcerative colitis model was induced by dextran sodium sulfate (DSS). Disease activity index scores were performed on colorectal tissue, blood in the stool, stool viscosity, etc., and the severity of inflammation was evaluated by HE staining on colorectal tissue. expression changes.

　　RESULTS: Compared with C57 mice, anti-Act1 mice had lower scores of colorectal inflammation indexes, less infiltration numbers of macrophages, and mRNA expressions of TNF-α, IL-1β and IL-6 after 7 days of DSS induction. lower.

　　Conclusion: Targeted inhibition of Act1 expression in macrophages can alleviate DSS-induced ulcerative colitis.