动物造模 z-bio 2022-03-31 405

　　Objective: To explore the route and method of hepatic artery cannulation in experimental rabbits.

　　METHODS: Thirty New Zealand white rabbits were randomly divided into 5 groups: peripheral intravenous injection, hepatic artery perfusion, hepatic artery lipiodol embolization, hepatic artery gelatin sponge particle embolization and direct liver puncture injection. Each rabbit was given clinically equivalent doses. elemene injection. The hepatic artery cannulation was performed using the Seldinger technique, and the auricular artery or the femoral artery was punctured according to the odd and even numbers of the experimental rabbits. The operation time, success rate and complications of puncture catheterization and hepatic artery catheterization were recorded. The venous blood of all experimental rabbits after administration was collected for pharmacokinetic analysis.

　　RESULTS: The success rates of puncturing the ear artery and femoral artery of rabbits were 55.6% (5/9, 2 on one side and 3 on both sides) and 88.9% (16/18, 12 on one side and 4 on both sides), respectively. The success rates of hepatic artery cannulation through the rabbit ear artery and femoral artery were 0% (0/5) and 100% (16/16), respectively, and the operation time was 28.4±13.6 and 33.9±19.6 min, respectively. Except for the direct liver puncture group and the femoral artery puncture group, 1 died due to anesthesia accident and 1 was euthanized after the failure of arterial puncture. After general anesthesia, the peripheral blood vessels of the experimental rabbits were obviously constricted, and it was difficult to collect blood several times in a short period of time through the rabbit ear vein.

　　Conclusion: The feasibility of hepatic artery cannulation through rabbit ear artery is low, and femoral artery cannulation is the first choice for interventional physicians. Blood sampling from rabbit jugular vein has inherent anatomical advantages and higher experimental value.