动物造模 z-bio 2022-03-31 251

　　OBJECTIVE: To evaluate the mutagenicity of the hydrolyzed liquid of clam meat, and to provide experimental data for the development and utilization of clam.

　　METHODS: Three mutagenicity tests, including Ames test, mouse polychromatic erythrocyte bone marrow micronucleus test and CHL cell in vitro chromosomal aberration test, were used to detect whether the clam meat hydrolyzate had mutagenic effect.

　　RESULTS: The number of retromuted colonies in each dose group of Chinese clam meat hydrolyzate in the Ames test was within the normal range, and did not exceed twice the number of spontaneous retromuted colonies. The results of 5 test strains were negative when S9 was added or not. Chromosomal aberration test of CHL cells in vitro showed that the chromosome aberration rate of each dose group of clam meat hydrolyzate was not significantly different from that of the negative control group (P>0.05), and the result was negative. In the mouse bone marrow micronucleus test, the micronucleus rate of each dose group was not significantly different from that of the negative control group (P>0.05), and the result was negative.

　　Conclusion: Under the conditions and scope of this experiment, there is no potential mutagenic effect of the clam meat hydrolyzate.