How to prepare a mouse model of Mycoplasma pneumoniae?

  (1) Replication method The Mycoplasma pneumoniae strain (ATCC29342) was cultured in SP-4 liquid medium at 37°C for 72 hours. After centrifugation, the pellet was prepared by SP-4 liquid culture and 1 x 100000000 CFU/ml. Two-month-old BALB/c mice were anesthetized by inhalation of methoxyhalothane. After over inhalation, 50μl of SP-4 liquid medium containing 1×100000000 CFU/ml mycoplasma pneumonia was administered intranasally. Confirm that depression will occur 1 to 2 days after vaccination.

  (2) Model features The onset of Mycoplasma pneumoniae infection is related to the adhesion of Mycoplasma pneumoniae. After adhering to epithelial cells, the host cells die, and then trigger a series of immune responses, leading to mild upper respiratory tract infections, leading to severe fatal pneumonia and various other diseases. Infection of animal mycoplasma pneumonia through the nasal cavity causes lung infection in rats. The infection route is similar to that of natural humans, but the amount of mycoplasma inoculation is not easy to control. The method is simple to operate, easy to obtain immune reagents, and refers to a large amount of immunological data, so the model is used to study the pathogenic mechanism of Mycoplasma pneumoniae and the immunopathological response to infection. It can be used for the evaluation of medication. Effects, screening of new drugs, etc.

  (3) Comparative medicine The main respiratory infections of mycoplasma pneumonia are pharyngitis, tonsillitis, and bronchitis. Only 3% to 10%, the lung lesions of the model animals are obvious, the entire lung is congested, and in severe cases, the lung lobe bleeding of mice is scattered with necrotizing lesions of various sizes and the clinical manifestations are also mild: pathological anatomy shows Bronchus, trachea, perivascular inflammatory exudate, solid pneumonia, tracheal exudate and many neutral and isolated inflammation sites. From the 21st day, the inflammatory response began to subside. Among people infected with Mycoplasma pneumoniae, the pathogen was still found in the respiratory tract even months after the disease was cured. The following situations may also occur in this model: TNF-α, IFN-γ, IL-6, IL-8, etc. in the tracheal lavage fluid of the model animals increase significantly, and it shows a state of high immune response.