动物造模 z-bio 2022-06-30 530

　　Objective: To explore the effects of different doses of icariin on rats with experimental IgA nephropathy, and to study its related mechanism.

　　Methods: A rat model of experimental IgA nephropathy was established, and the experiment was divided into 5 groups, normal control group (con), modeling group (IgA), icariin low-dose group (ICA-L), icariin medium Dose group (ICA-M) and high-dose icariin group (ICA-H); after corresponding treatment in each group, the levels of urine erythrocytes, urine protein and urine NAG were determined; immunofluorescence staining was used to detect the precipitation of IgA ; Immunohistochemical staining and real-time quantitative PCR were used to detect the protein expression levels of NF-κBp65 and MCP-1 and the mRNA expression changes of IL-4, IL-10 and IL-13.

　　Results: After treatment with icariin, the levels of urinary red blood cells, urinary protein and urinary NAG and IgA precipitation in rats with experimental IgA nephropathy were reduced, and the protein expression levels of NF-κBp65 and MCP-1 and IL-4 were also reduced. mRNA expression of IL-10 and IL-13.

　　Conclusion: Icariin has a certain curative effect on experimental IgA nephropathy, which may be related to regulating the expression of NF-κBp65, MCP-1 factor and immune regulation.