动物造模 z-bio 2022-10-22 433

　　Objective: This article investigates the effect of epithelial sodium channel (ENaC) on the function and activity of osteoclasts.

　　Methods: Rat bone marrow monocytes were induced to differentiate into osteoclasts by using rat macrophage colony-stimulating factor and nuclear transcription factor-κB receptor activator ligand. The cells were inoculated into 12-well plates at a density of 1.5×104, and 3 wells were divided into 1 group according to the random table, and divided into 4 groups: Control group and different concentrations of amiloride (Ami, an inhibitor of ENaC) group. Tartrate-resistant acid phosphatase (TRAP) staining was used to identify positive osteoclasts; osteoclasts and bone slices were co-cultured to determine the number of bone resorption lacuna; RT-PCR was used to analyze the osteoclast marker enzyme gene cathepsin K ( CK) expression.

　　RESULTS: After different concentrations of Ami treated osteoclasts, TRAP staining-positive osteoclasts decreased, inhibited the formation of osteoclasts and bone resorption, and decreased the expression of osteoclast-specific gene CK.

　　Conclusion: This experiment proves that ENaC is expressed on osteoclasts at the cellular level and regulates the differentiation and bone resorption of osteoclasts, indicating that ENaC may be involved in the function regulation of osteoclasts, suggesting that there may be a regulation related to ENaC in osteoclasts. It provides a new idea for the study of bone metabolism.