动物造模 z-bio 2023-03-20 487

　　Objective: To use 16S rRNA high-throughput sequencing method to determine the oral flora of several commonly used experimental animals (Tibetan miniature pigs, beagle dogs, macaques, New Zealand rabbits, Wistar rats), and to compare and analyze the oral flora of humans. Provide basic data for animal model study of oral microecology.

　　METHODS: Oral flora specimens from Tibetan miniature pigs, beagle dogs, macaques, New Zealand rabbits, Wistar rats and humans were collected with disposable cotton swabs, and total DNA was extracted from the samples, and the 16S rRNA V4 region was amplified using tagged universal primers. Fragments, Illumina sequencing, BIPES and QIIME analysis to compare the diversity and structure of bacterial flora.

　　RESULTS: There were significant differences in the abundance of oral flora between humans and five commonly used experimental animals (P<0.05). Different species of animals had their own unique oral flora, but the oral flora of monkeys was the most similar to that of humans.

　　Conclusion: According to the degree of similarity with a certain type of oral flora of humans, among the five animals, the levels of Fusobacterium and Porphyromonas in the oral cavity of monkeys are the most similar to those of humans, suggesting that monkeys may be the study of human oral bacteria. Group of more suitable model animals. From the perspective of specific bacterial phylum, Tibetan miniature pigs may be a more suitable model animal to study diseases related to Proteobacteria; Beagle dogs may be a more suitable model animal to study diseases related to Spirochaetes.