动物造模,膝骨关节炎 z-bio 2022-10-22 660

　　Objective: To observe the effect of short needling method on the expression of sex-determining region (Sox9), vascular endothelial cell growth factor (VEGF) and type X collagen (ColⅩ) in articular chondrocytes of rabbit knee osteoarthritis (KOA) articular cartilage cells, and to explore the effect of short needling method. Mechanism of action in the treatment of KOA.

　　Methods: 40 New Zealand rabbits were randomly divided into 10 normal group (N group) and 30 modeling group according to the random control table. Hulth operation was performed on the modeling group to replicate the knee osteoarthritis model. After successful modeling, they were randomly divided into control group (M group), short needling group (CN group) and ordinary acupuncture group (NTN group). The unilateral Zusanli, inner and outer knee eyes, Liangqiu and Yinlingquan points of the left knee joint were taken, and the needles were retained for 20 min, once a day, for a course of 5 days, for a total of 4 courses of treatment, which were randomly selected before and after treatment. The behavioral changes of New Zealand rabbits in each group were observed; the general morphology and histological score of cartilage were observed after the experiment; the expressions of Sox9 and ColX proteins were detected by Western-Blot; the expressions of VEGF and ColX proteins were detected by immunohistochemistry; Sox9 was detected by RT-PCR and VEGFmRNA expression.

　　Results: The behavior of rabbits and the general observation of cartilage in M, CN, and NTN groups changed significantly before and after treatment; HE staining showed that cartilage tissue damage in CN and NTN groups was alleviated, and there was a tendency to repair; N, M, CN groups There were significant differences in cartilage Mankin score between group and NTN group (P<0.01). Compared with group M, CN group and NTN group had higher Sox9 protein and mRNA expression, lower VEFG protein and mRNA expression, and lower ColⅩ protein expression (P<0.01). ); the difference between CN group and NTN group was statistically significant (P<0.01).

　　Conclusion: Short needling method can effectively treat KOA, and its mechanism may be to increase the content of Sox9 in chondrocytes, reduce the expression of VEGF and ColⅩ, and inhibit the differentiation of mast cells, thereby maintaining the normal phenotype of chondrocytes and repairing cartilage.