动物造模,肺成纤维 z-bio 2022-05-26 702

　　Objective: To investigate the effects of IL-27 and its receptor (WSX-1) on the proliferation, transformation and collagen synthesis of mouse lung fibroblasts induced by TGF-β1.

　　Methods: The IL-27R/pCDNA3.1 overexpression vector was constructed. The experiment was divided into 6 groups: normal group, TGF-β1 group, IL-27 group, IL-27 receptor group, IL-27+IL-27 receptor group, TGF-β1+IL-27+IL-27 receptor group. body group. The growth of the cells was observed under a light microscope, and the effect of each treatment group on the proliferation of lung fibroblasts was detected by MTT. RT-PCR and Western blotting were used to detect the expression of myofibroblast marker a-SMA and collagen type I and III, and immunofluorescence was used to detect the localization and expression of a-SMA protein.

　　Results: (1) Both IL-27 and IL-27R could inhibit the proliferation of lung fibroblasts; (2) TGF-β1 increased the expression of α-SMA in lung fibroblasts, and promoted the synthesis of collagen I and III in lung fibroblasts (3) IL-27 and its receptors can inhibit the expression of α-SMA in lung fibroblasts and reduce the synthesis of collagen I and III in lung fibroblasts; (4) When IL-27/IL-27R acts together , had no effect on the proliferation and transformation of lung fibroblasts.

　　Conclusion: IL-27 or IL-27 receptor can inhibit the proliferation, transformation and collagen synthesis of mouse pulmonary fibrotic cells induced by TGF-β1, but the coexistence of IL-27 and IL-27 receptor cannot. It exerts an inhibitory effect, which may be due to the fact that the neutralization of exogenous IL-27 and IL-27 receptor cannot activate cell-related signals.