动物造模 z-bio 2022-08-18 804

　　Objective: To explore the possible mechanism of exogenous β-NGF on bone remodeling during bone defect healing.

　　Methods: Surgically excised the left and right sides of the skull top of SD rats, and established a model of continuous perfusion of β-NGF in the standard bone defect of the paired rat skull. The expression level of 2 and special staining technique (TRAP staining) were used to detect the expression level of tartrate-resistant alkaline phosphatase in the bone defect area, and the integral optical density (IOD) of the two were measured by the image processing software IPP6.0 to explore the BMP. The possible mechanism of -2 and osteoclast activity in the regulation of new bone formation and remodeling by β-NGF.

　　Results: The positive expression level of BMP-2 immunohistochemical staining in the experimental group was significantly higher than that in the control group on the 14th day, and the difference was significant (P<0.05). The three time points of 7, 21, and 28 days were significantly lower than those of the control group, and the difference was significant (P<0.05).

　　Conclusion: Exogenous β-NGF has an important regulatory role in the process of bone defect repair, which may inhibit bone resorption by promoting the expression of BMP-2 and inhibiting the activity of osteoclasts.