动物造模 z-bio 2022-10-26 659

　　Objective: To observe the effects of DEHP exposure on placental growth and development, the number of uNK cells at the maternal-fetal interface and angiogenesis in mice by exposure to di(2-ethyl)hexyl phthalate (DEHP) during pregnancy.

　　Methods: From the 1st day of pregnancy, different doses of DEHP 125, 250, 500 mg·kg-1·d-1 were given by in vitro forced gavage method, and the uterus and placenta samples were taken and weighed on the 13th day of pregnancy. , and its effects on the growth and development of mouse placenta, the number of uNK cells at the maternal-fetal interface and the formation of blood vessels were observed by HE staining and immunohistochemistry.

　　Results: (1) Compared with the control group, low-dose DEHP125 and 250 mg·kg-1·d-1 had no significant effect on embryo implantation number (P>0.05). However, 500 mg·kg-1·d-1 DEHP in the high-dose group significantly decreased the number of embryo implantations (P<0.05). In addition, DEHP exposure can significantly increase the fetal abortion rate; (2) Compared with the control group (0.0786±0.0143 g), DEHP decreased placental weight in a dose-dependent manner of 125, 250, 500 mg·kg-1·d-1 (respectively: 0.0637±0.0133, 0.0587±0.0176, 0.0524±0.0183 g; P<0.01), and significantly reduced the total area of the placenta and the area of trophoblast cells in the cavernous layer; (3) Compared with the control group, DEHP exposure decreased It led to a significant decrease in the number of fetal blood vessel branches, blood vessel collapse and atresia; (4) Compared with the control group (105.1±14.2/HP), DEHP exposure dose-dependently inhibited the number of uNK cells (respectively: 83.2±10.3, 60.7± 12.4, 50.4±14.5/HP; P<0.01).

　　Conclusion: DEHP exposure during pregnancy can significantly inhibit the growth and development of the placenta, which may be by affecting the angiogenesis and the number of uNK cells at the maternal-fetal interface.