动物造模,止血带性肢体缺血再灌注损伤 z-bio 2022-12-28 776

　　Objective: To investigate the changes of platelet mitochondria after tourniquet ischemia-reperfusion injury, and to provide guidance for the clinical application of tourniquet.

　　METHODS: Thirty SD rats were randomly divided into control group (6 rats) and ischemia-reperfusion group (24 rats), and then randomly divided into 4 groups. Blood samples were collected at 2, 6, 12, and 24 h after reperfusion respectively. ). A self-made tourniquet was used to surround the base of the rabbit's thigh (pressure 280 mmHg) to simulate tourniquet ischemia, and the tourniquet was released after 4 hours. Blood was collected at corresponding time points to separate platelets. The number of platelets was determined by automatic blood cell counter; the ATP content of platelets was determined by luciferin-luciferase kit; the changes of mitochondrial membrane potential (△ym) were detected by JC-1 mitochondrial membrane potential kit; platelets were detected by cytochrome C apoptosis kit Cytoplasmic cytochrome C content; lipid peroxide kit to detect lipid peroxide in platelets.

　　Results: Compared with the control group, the content of ATP in platelets in the ischemia-reperfusion injury group was significantly decreased at 2 and 6 h (P<0.05 or P<0.01), while the content of platelets with low mitochondrial membrane potential, the content of cytochrome C, The lipid peroxide content gradually increased at 2 and 6 h and had statistical differences (P<0.05 or P<0.01); at 12 h, the above changes were recovered but still statistically significant (P<0.05 or P<0.01). <0.01); 24="" p="">0.05). There was no statistical difference in platelet count between the two groups (P>0.05).

　　Conclusion: Tourniquet ischemia-reperfusion injury can lead to the impairment of platelet mitochondrial function, which mainly occurs in the early stage of ischemia-reperfusion (6 h), and gradually recovers in the later stage, but has no significant effect on the number of platelets.