动物造模 z-bio 2022-06-30 473

　　Objective: To evaluate the preventive effect of sodium tanshinone IIA sulfonate (STS) on postoperative peritoneal adhesions in experimental rats and to explore its mechanism.

　　METHODS: A rat postoperative peritoneal adhesion model was established. 75 rats were randomly divided into model group, STS high-dose group, middle-dose group, low-dose group, and blank group, with 15 rats in each group; STS high-dose, middle- and low-dose groups were administered at 20, 10, and 5 mg/dose, respectively. kg STS, and the model control group was given an equal volume of normal saline by intraperitoneal injection for 7 consecutive days. Rats in each group were sacrificed 7 days later, the adhesion grade was evaluated, the level of tPA/PAI-1 in the tissue was detected by ELISA, the activity of tPA in the peritoneal fluid was detected by the chromogenic substrate method, and the TGF-β1 and Collgen-1 in the peritoneal tissue were detected semi-quantitatively by IHC. Expression of I; wound healing strength test to investigate whether STS affects wound healing.

　　Results: Compared with the model group, the STS high, medium and low dose groups all reduced the occurrence of peritoneal adhesions; tPA activity in peritoneal fluid was significantly increased, tPA/PAI-1 protein levels in tissue were significantly increased, TGF-β1 and Collgen-I Protein expression was significantly reduced. Meanwhile, intraperitoneal injection of STS did not affect wound healing.

　　Conclusion: Intraperitoneal injection of STS can effectively prevent the occurrence of postoperative adhesions, and its mechanism may be by reducing the expression of TGF-β1, down-regulating its activation of TGF-β/Smads pathway, and reducing the level of downstream PAI-1, thereby up-regulating the activity of peritoneal fibrinolysis system. realized.