动物造模 z-bio 2022-09-26 679

　　Objective: To study the effect of minocycline on the hyperpolarization-activated current (Ih) of colloidal (SG) neurons in the spinal dorsal horn of rats.

　　METHODS: Male SD rats aged 3-5 weeks were selected to make transverse sections of the isolated spinal cord. Whole-cell patch clamp technique was used to record SG neurons for 1 h, perfused with different concentrations of minocycline (1-300 μmol/L) and observed. Effect on Ih.

　　RESULTS: About 50% of the recorded SG neurons could record Ih, and this current was blocked by the Ih blockers CsCl and ZD7288. Minocycline can reduce Ih amplitude and Ih current density. This effect is reversible and dose-dependent, and the median effective inhibitory concentration (IC50) is 34 μmol/L.

　　Conclusion: Minocycline can inhibit the Ih of SG neurons in the dorsal horn of the spinal cord, thereby reducing the excitability of SG neurons, which is of great significance for the regulation of pain.