动物造模,糖尿病大鼠心肌纤维化 z-bio 2022-04-08 542

　　Objective: To investigate the effect of gas signal molecule hydrogen sulfide (H2S) on myocardial fibrosis and MAPK1/3 and MMP-8 protein expression in diabetic rats.

　　Methods: Forty SD rats were randomly divided into 4 groups: normal control group, diabetic rat group (STZ group), hydrogen sulfide intervention diabetic rat group (STZ+H2S group) and hydrogen sulfide intervention normal rat group (H2S group) ). The diabetic rat model was established by intraperitoneal injection of streptozotocin (STZ) (40 mg/kg); the control group was intraperitoneally injected with normal saline every day; the STZ+H2S group and the H2S group were intraperitoneally injected with sodium hydrosulfide (H2S donor) solution every day (100 μmol/kg), the rats were sacrificed after 8 weeks, the pathological changes of myocardial fibers were observed by HE staining, and the expressions of Collagen I, MAPK1/3 and MMP-8 proteins were detected by Western blot.

　　Results: Compared with the control group, the myocardial tissue collagen content in the diabetic group was significantly increased, the myocardial interstitial fibrosis, and the protein expressions of Collagen I, MAPK1/3 and MMP-8 were significantly increased (P<0.05). After hydrogen sulfide intervention in mice, most myocardial fibers were arranged in parallel, with clear structure, and there was a small amount of loose connective tissue in the interstitium. The protein expressions of Collagen I, MAPK1/3 and MMP-8 were significantly down-regulated (P<0.05). the="" degree="" of="" myocardial="" fibrosis="" and="" tissue="" collagen="" p="">0.05 in the hydrogen intervention group.

　　Conclusion: H2S can improve myocardial fibrosis in diabetic rats, and its mechanism may be related to the inhibition of MAPK1/3/MMP-8 signaling pathway.