动物造模 z-bio 2022-07-12 443

　　Objective: To establish a practical animal model of chronic metabolic diseases, and to explore the correlation between hyperuricemia and glucose and lipid metabolism, as well as its pathological damage mechanism.

　　Methods: The high-fat and high-hypoxanthine diet combined with potassium oxonate was used to establish the model to detect the dynamic changes of serum uric acid and triglyceride in quails. Arteries were examined for pathology.

　　RESULTS: High-fat and high-hypoxanthine diet + potassium oxonate induced significantly increased serum uric acid and triglyceride levels in quails. The glucose tolerance test showed that the quails in the combined model group had higher peak blood glucose, the area under the blood glucose curve was larger than that of the other groups, and the insulin level was also significantly higher than that of the other groups. The combined modeling group can also increase the activity of serum aspartate aminotransferase and alanine aminotransferase, as well as the contents of blood urea nitrogen and creatinine, which have obvious damage effects on the liver, aorta and kidneys. These damage effects can be affected by bezafibrate and febuxostat. relieved to varying degrees.

　　Conclusion: The combined induction of high-fat and high-hypoxanthine diet + potassium oxonate can establish a simple, stable and durable animal model of chronic metabolic diseases.