动物造模 z-bio 2022-11-21 647

　　Objective: To investigate the protective effect of Atractylodes Rhizoma extract on gastric mucosa and its effect on gastrointestinal immune function in spleen deficiency model rats, and to clarify the mechanism of ERA's intervention in spleen deficiency syndrome.

　　Methods: The rat model of spleen deficiency syndrome was established by feeding Xiaochengqi Decoction decoction and abnormal hunger and satiety. After the model was successfully replicated, the animals were randomly divided into spleen deficiency model group, ERA high, medium and low dose groups, and domperidone group. Continuous intragastric administration for 10 d. The intestinal perfusion method was used to detect the content of IgA in the intestinal perfusate of the rats, the blood collection method of the abdominal aorta was used to detect the content of IgG in the serum of the rats, and the indexes of the thymus and spleen of the rats were determined. The blood flow in the gastric mucosa of rats was measured by a microcirculation flowmeter, and the expression levels of TFF1 and colon TLR4 in the gastric mucosa of the rats were determined by immunohistochemistry.

　　Results: Compared with the normal group, the gastric mucosal morphology, gastric mucosal blood flow, and related immunological indexes of the rats in the model group were significantly changed; The expression of trefoil factor 1 (TFF1) in mucosal tissue, IgA in intestinal perfusate, serum IgG content, thymus, spleen index and colon TLR4 expression increased in different degrees, and the differences were statistically significant (P<0.05 or P<0.01).

　　Conclusion: ERA can inhibit gastric mucosal damage in rats with spleen deficiency syndrome, protect and repair damaged mucosal tissue, and improve the immune function of rats with spleen deficiency syndrome.