动物造模,压力性尿失禁大鼠模型 z-bio 2022-09-24 485

　　Objective: To study the effect of IGF-1 gene therapy on the rat model of postpartum stress urinary incontinence, and to explore the ideal treatment method for the disease.

　　METHODS: 240 SD female mice were modeled by hydrocystovaginal dilation, 148 were randomly selected from 185 successful models and divided into 5 groups, which were treated with gene therapy of IGF-1, drug therapy with ammonia, electrical stimulation, Empty plasmid vector injection and no treatment, 20 unmodeled rats were selected as blank control, and urodynamics and serum biochemical indexes (LDH, CK) were detected in each group at 1, 21, 42 and 63 days after treatment, respectively. etc., and observe the changes of the muscle fibers of the pubococcygeus muscle under the light microscope.

　　Results: At 21 days after treatment, no matter the maximum bladder capacity, leakage point pressure or contractility/muscle weight ratio, the IGF-1 group and the electrical stimulation group had better therapeutic effects; There was no significant difference between groups (P>0.05).

　　Conclusion: The effect of IGF-1 gene therapy and electrical stimulation therapy on the rat model of postpartum stress urinary incontinence is better than that of other groups such as drug therapy.