动物造模 z-bio 2022-09-24 543

　　OBJECTIVE: To investigate the effect of a high-fat diet of thulia oil rich in n-3 polyunsaturated fatty acids on insulin sensitivity and the expression of related genes in insulin-resistant rats.

　　Methods: The insulin resistance model rats were randomly divided into two groups: high fat group (HF) and perilla oil group (PO). PO was 20% of the ratio of perilla oil to lard in HF. %, after 4 weeks, the insulin sensitivity of the rats was determined; the contents of sesame oil and serum α-linolenic acid (ALA) of the rats were detected by gas chromatography; the skeletal muscle glucose transporter 4 (glucose transporter 4) was detected by Western blot 4, GLUT4) and insulin receptor substrate-1 (insulin receptor substrate-1, IRS-1) protein expression; skeletal muscle Glut4, Irs-1 mRNA expression was detected by real time PCR.

　　Results: After 4 weeks of Suzi oil intervention, compared with HF group, with the increase of ALA intake and serum ALA content in PO group, GLUT4 protein and mRNA expression were significantly increased, while IRS-1 protein and mRNA were significantly increased. The mRNA expression was significantly decreased; the results of the hyperinsulinemic-euglycemic clamp test showed that there was no significant difference in insulin sensitivity between the two groups of rats.

　　Conclusion: ALA (0.556 g/d) high-fat diet intervention can regulate the expression of GLUT4 and IRS-1 in skeletal muscle, but cannot improve insulin sensitivity in insulin-resistant rats.