动物造模 z-bio 2022-04-09 675

　　OBJECTIVE: To explore the degree of damage to animal welfare caused by routine experimental operations and the effect of music intervention.

　　Methods: 3-week-old male ICR mice were used, and the experimental operation group were treated with Baoding (E-r), gavage (E-g), intraperitoneal injection (E-p) and orbital venous plexus blood collection (E-o) respectively, and the music intervention group was treated with the same treatment and then given 1h musical intervention (M-r, M-g, M-p, M-o). After 7 days of continuous treatment, neuroendocrine, immune, biochemical, growth indicators and feed energy efficiency ratios related to welfare were determined.

　　Results: ①Compared with the control group, the body weight gain and feed energy efficiency ratio of the four experimental operation groups were significantly decreased; TG, HDL-C, WBC, IL-2, β-EP, spleen and thymus indexes were significantly decreased in the E-r group. CORT, ACTH and EPI were significantly increased; TG, WBC, IL-2 and β-EP were significantly decreased in E-g group, CORT, ACTH and EPI were significantly increased; IL-2 was significantly decreased in E-p group, CORT, ACTH and EPI were significantly increased In the E-o group, the spleen index and WBC were significantly decreased, and the CORT was significantly increased. ②Compared with the experimental operation group, the body weight gain, feed energy efficiency ratio, spleen index and IL-2 in the M-r group were significantly increased, while CHOL, CORT, ACTH and β-EP were significantly decreased; the WBC was significantly increased, and the CORT was significantly decreased in the M-g group WBC decreased significantly in M-p group; feed energy efficiency ratio increased significantly in M-o group.

　　Conclusion: The four routine experimental operations all caused different degrees of damage to the health and welfare of mice, and music intervention can improve the degree of operation stress and welfare impairment in mice.