动物造模 z-bio 2022-04-14 442

　　OBJECTIVE: To genetically screen zebrafish mutants with defective liver, intestine and gallbladder development.

　　METHODS: Wild-type zebrafish were mutagenized with ENU and the classical F2 generation screening was performed. Whole embryo in situ hybridization with lfabp as a probe and BES-H2O2-Ac fluorescent dye were used to detect the phenotypes of early embryonic liver, intestine and gallbladder of zebrafish respectively. .

　　RESULTS: Twenty-three zebrafish digestive organ development-deficient mutant strains derived from 14 F2 families were screened out of 128 mutant genomes, and they were divided into 6 types according to phenotype.

　　Conclusion: The developmental regulatory mechanisms of zebrafish liver, intestine and gallbladder have similarities and differences.