动物造模 z-bio 2022-12-15 373

　　Objective To explore the relationship between the body's adaptability to exercise fatigue and the expression of monocarboxylate transporter in cerebral cortex.

　　Methods Forty-two clean-grade SD rats were randomly divided into control group and fatigue groups 1-6. Rats in fatigue groups 1-6 were subjected to treadmill load-to-failure exercise for 1, 3, 5, 7, 9, and 14 d, respectively, to establish exercise fatigue models. Firstly, the average time for the rats in each fatigue group to reach the state of exhaustion was recorded to determine the changing trend of the rats' exercise fatigue. Subsequently, the expression changes of MCT1, MCT2 and MCT4 in the motor cortex of the rats in each group were detected by western blotting and immunofluorescence double-labeling method.

　　Results After 5 days of exercise, the fatigue time of rats in the three groups was the shortest [(67.00±7.07) min], and it recovered to (89.17±9.45) min after 9 days of exercise, and the difference was statistically significant (P<0.05). ; After that, the motor ability of rats remained basically constant. Western blotting showed that MCT1, MCT2 and MCT4 were slightly expressed in the motor cortex of the rats in the control group; after 5 d of exercise, the expression of MCT2 in the fatigue group 3 was increased by 74.2% compared with the control group (P<0.05); after 7 d of exercise Compared with the control group, the expressions of MCT1 and MCT2 in fatigue 4 groups increased by 89.5% and 92.0% (P<0.05). The results of immunofluorescence and western blotting were consistent.

　　Conclusion The regulation of brain lactate metabolism represented by the increased expression of MCTs in the cerebral cortex is related to the body's adaptability to exercise fatigue, and may be the target of medical intervention for exercise fatigue.