动物造模 z-bio 2022-05-05 271

　　Objective To make a simple fixation device for acupuncture on the hindlimb of mice, and to provide an economical, practical, convenient and efficient tool for acupuncture experimental operation.

　　Methods The mouse hindlimb immobilizer was made according to the design drawing. Four experimenters A, B, C, and D respectively used cloth mouse clothing and mouse hindlimb immobilizer to immobilize the mice. time, the number of mouse hindlimb retraction, the number of times the needle fell off, and the weight of the mouse to compare the immobilization effect of the two in acupuncture operation and the effect on the stress response of the mouse; in addition, through the thermal radiation pain threshold detection, open field test , Elevated plus maze experiment to observe the effects of different fixation methods on pain threshold, cognition, emotion and so on in mice.

　　Results The mouse hindlimb immobilizer was simple to make. Compared with the cloth mouse coat, there was no significant difference in the weight of the mice. However, using the mouse hindlimb immobilizer to immobilize mice for acupuncture was more efficient. The number of times of retraction and needle falling off was less; there was no significant difference in the indicators of thermal radiation pain threshold detection, open field test and elevated plus maze test between groups.

　　Conclusion The mouse hindlimb immobilizer is easy to use and helps to improve the experimental efficiency. It has no obvious effect on the pain threshold, cognition and emotion of mice, and can be used according to specific experimental needs.