动物造模,肺间充质干细胞 z-bio 2022-09-21 402

　　Objective To establish a stable technique for the isolation, culture and identification of mouse lung mesenchymal stem cells, and to study the mechanism of lung tissue repair and regeneration using mouse lung mesenchymal stem cells.

　　Methods The lungs of mice were aseptically isolated, and the lung mesenchymal stem cells were isolated and cultured by collagenase digestion and tissue adherence method. The cell morphology and growth characteristics were observed, and cell surface markers were analyzed by flow cytometry.

　　Results Both methods successfully isolated and cultured pulmonary mesenchymal stem cells. Flow analysis showed that Sca-1, CD90, CD29 and CDCD44 were all highly expressed, while CD31 and CD45 were all lowly expressed. After in vitro induction, lung mesenchymal stem cells can differentiate into adipocytes and osteoblasts.

　　Conclusions In this study, mouse lung mesenchymal stem cells were isolated and cultured by two methods, and the cells obtained by the two methods had similar surface markers of mesenchymal stem cells and possessed the ability of adipogenic and osteogenic differentiation.