动物造模,1型糖尿病 z-bio 2022-05-06 447

　　Objective To investigate the function of nitrergic and cholinergic nerves in jejunal smooth muscle and the morphological changes of jejunum in early stage type 1 diabetic rats.

　　Methods Male SD rats were randomly divided into normal control group and diabetic model group, and a diabetic rat model was established by intraperitoneal injection of streptozocin (STZ) 60 mg/kg. After 4 weeks, the neurogenic contraction response was induced by the application of the field stimulation, and the contraction response of the isolated jejunal smooth muscle, as well as the functional changes of the nitrergic and cholinergic nerves were observed by the isolated organ muscle tone analysis method; HE was performed on the paraffin tissue section. The morphological changes of jejunum were observed by staining and immunohistochemical detection.

　　Results Compared with normal rats, jejunal intermuscular cholinergic nerve function was impaired in STZ-induced early diabetic rats, but no significant changes were found in nitrogenergic nerve function. The increased layer thickened, the goblet cells decreased, and the ratio of villus length to the depth of the intestinal crypts (chorionic-gland ratio) decreased.

　　Conclusion In the early type 1 diabetes rat model 4 weeks after STZ permeabilization, the neurological function and morphology of the jejunum tissue have changed significantly, suggesting that the intestinal tract, as a sensitive organ of diabetes, plays an important role in the early diagnosis and prevention of diabetes. Clinical reference significance.