动物造模 z-bio 2022-09-29 478

　　Objective To explore the feasibility and application value of testing experimental materials in SPF laboratory mouse barrier facilities.

　　Methods The experimental materials brought into the barrier facility were collected, the non-cellular samples were cultured, and the cell samples were tested for Mycoplasma by PCR.

　　Results A total of 14 708 experimental materials were collected in 5 years, including 13 085 non-cellular samples, with an average detection rate of 1.99% for bacteria; 1 623 cases of cell samples, with an average detection rate of 4.81% for mycoplasma. On the whole, the positive detection rate has shown a downward trend year by year, and has stabilized at a relatively low level in recent years.

　　Conclusion The feasibility of large-scale continuous inspection of experimental materials brought into barrier facilities can prompt researchers to strengthen the aseptic handling of animal experimental materials, and allow managers to determine the degree of harm from experimental materials. One of the effective measures for infection prevention and control.