动物造模,单纯疱疹病毒Ⅱ型 z-bio 2022-10-10 475

　　Objective To compare the pathological changes of BALB/c mice infected with herpes simplex virus type Ⅱ (HSV-2) by different routes.

　　METHODS: BALB/c mice were infected with HSV-2 in three different ways of transvaginal, nasal cavity and cornea respectively. viral load.

　　Results The mice in the nasal cavity group had no obvious symptoms, but only had a survival rate of 27.27% by the end of the observation period; the mice in the vaginal group showed symptoms of varying degrees of vulvitis and weight loss. The survival rate of the mice was observed 8 days after infection. It was 0; the mice in the cornea group showed different degrees of keratitis, and the survival rate at the end of the observation period was still 63.64%. The results of pathological changes showed that only the brain tissue of the mice in the nasal cavity group had obvious pathological changes; the mice in the vaginal group and the cornea group had obvious pathological changes at the challenge site, but the mice in the vaginal group had obvious pathological changes in the brain and spinal cord. The results of viral load detection in nerve tissue showed that compared with the control group, the viral load of HSV-2 in the brain and spinal cord of mice in the three experimental groups was significantly increased, and increased with the increase of infection time.

　　Conclusion HSV-2 infection in BALB/c mice by three different routes can cause different degrees of pathological changes in mice. This study provides a theoretical basis for the study of the pathogenesis of neurological diseases caused by HSV-2 infection.