动物造模 z-bio 2023-06-06 743

　　Objective To establish a rat model of external jugular vein catheter-related thrombosis (CRT), to observe the formation of CRT in rats, and to detect serum tissue factor (TF), thrombomodulin (TM) and von Willebrand disease in rats. Factor (vWF) content, to study its relationship with the formation of CRT.

　　Methods A total of 120 male SD rats were randomly divided into blank control group ( n = 40 ) , sham operation group ( n = 40 ) and model group ( n = 40 ). Eight rats were randomly selected on 7 d, 10 d and 14 d, pathological sections were used to observe the thrombosis of the rats, and serum TF, TM and vWF contents were detected by ELISA.

　　Results There was no thrombosis in the blank control group and the sham operation group, and 34 rats in the model group developed CRT, and the incidence of CRT was 85%. The content of TM in rats with CRT in the model group was significantly higher than that in the rats without CRT (P<0.01), and the content of TM in rats without CRT in the model group was significantly higher than that in the sham operation group (P<0.01) , the contents of TM and vWF in the model group without CRT were significantly higher than those in the blank control group (P<0.01). At each time point after operation, the contents of TF and TM in the model group were significantly higher than those in the blank control group and the sham operation group (P<0.01). At 4 d, 7 d, 10 d, and 14 d after operation, the vWF content in the model group was significantly higher than that in the blank control group and the sham operation group (P<0.01). With the prolongation of observation time in the model group after operation, the serum TF, TM and vWF contents of rats gradually increased with time, and the TF and vWF contents increased sharply at 1-4 days after operation, reached the peak at 10 days after operation, and then decreased sharply. ; TM content increased the most at 4-7 days after operation, reached the peak at 7 days after operation, and then began to decrease.

　　Conclusion The formation of CRT in rats is closely related to the abnormal expression of TF, TM and vWF in serum.