动物造模,基因敲除 z-bio 2022-05-07 641

　　Objective To analyze the breeding and identification of Ifnar knockout mice, and to provide an ideal animal model for the study of various viruses.

　　Methods The introduced homozygous Ifnar knockout mice were reared and bred in a cage of 1 male and 2 females. The mouse tail genomic DNA was extracted from the offspring, the target gene fragment was amplified by PCR, and then subjected to agarose gel electrophoresis. Genotyping results.

　　Results Ifnar knockout mice were successfully bred, a batch of knockout mice were obtained, and Ifnar knockout homozygous mice were successfully identified by PCR.