动物造模 z-bio 2022-05-31 541

　　Objective To study the effect of dehydroepiandrosterone (DHEA) on superovulation and oocyte quality in C57BL/6J female mice and its mechanism.

　　Methods Thirty 3-week-old C57BL/6J female mice were randomly divided into 3 groups, namely control group, low-dose treatment group (2 mg/kg·d-1) and high-dose treatment group (5 mg/kg·d-1). After 1 week of intragastric administration of DHEA, in vitro fertilization and collection of cumulus cells were performed after superovulation. The RNA of cumulus cells was extracted for qRT-PCR to detect the mRNA levels of embryo quality-related genes Grem1, Has2, Ptgs1, Ptgs2 and Vcan and the mRNA levels of apoptosis-related genes Caspase3 and Bcl2l10.

　　Results After superovulation in DHEA-treated female mice, in vitro fertilization (IVF) showed that the proportion of heterozygous eggs in the experimental group decreased. Compared with the control group, the transcript levels of Has2, Ptgs1 and Vcan related genes related to embryo quality in the cumulus cells of the experimental group were up-regulated, while the mRNA levels of Germ1 and Ptgs2 were down-regulated. The mRNA level of Caspase3 was down-regulated and the mRNA level of Bcl2l10 was up-regulated.

　　Conclusion After DHEA treatment, the quality of oocytes can be improved by inhibiting cumulus ketocytosis.