动物造模 z-bio 2022-05-09 495

　　Objective In order to improve the monitoring and epidemiological investigation of the entry and exit wild and experimental primate monkey T lymphocyte tropism virus type 1 infection at the port, this study established the recombinase polymerase amplification (RPA) and fluorescent RPA. Methods for detection of STLV-1.

　　Methods The gag polyprotein gene sequence of STLV-1 isolated from different countries and regions was analyzed by software, RPA primers and probes were designed, and RPA and fluorescent RPA methods were established to detect STLV-1. properties and stability are verified.

　　Results By comparing the detection of other specific pathogens in monkeys with STLV-1, it was confirmed that the established detection method had good specificity. 1 The detection of positive and negative nucleic acid samples confirms that the established RPA and fluorescent RPA methods have the same stability and reliability as PCR methods.

　　Conclusion The RPA and fluorescent RPA methods established in this study have good specificity, sensitivity and reliability for STLV-1 detection, and can be applied to STLV-1 surveillance and epidemiological investigation.