【Animal Modeling】-Results and analysis of the ability to detect Bordetella bronchus in experimental animals

  Objective To carry out proficiency testing activities in 2017, and evaluate the detection ability of domestic laboratory animal testing laboratories for Bordetella bronchiscus.

  Methods According to relevant CNAS documents, China National Institute for Food and Drug Control, as the provider of proficiency testing, prepared comparative samples containing Bordetella bronchiscus, Pasteurella aerogenes and Escherichia coli as interfering bacteria, and provided them to participating laboratories. Intraocular feedback test results and reports when specified.

  Results The average bacterial content of the three prepared samples was greater than 1x108CFU/mL, and the accelerated stability time at 37℃ was not less than 20d, which met the comparison requirements. A total of 25 laboratory animal testing laboratories in 18 provinces and cities across the country participated, and all of them reported results and reports on time, with a satisfaction rate of 88%.

  Conclusion This proficiency test lays the foundation for the further development of the experimental animal respiratory tract bacteria comparison project. Most participating laboratories have consistent and reliable detection capabilities for B. bronchialis.