动物造模,圆环病毒2型 z-bio 2022-12-28 527

　　Objective Whether circovirus 2 can infect human cells has been a topic of debate. In this study, RNA sequencing (RNA-seq) technology was used to analyze the differentially expressed genes and their related signaling pathways and biological functions after PCV2 infection of human cells, in order to provide human cells with antiviral effects on PCV2 and other viruses. research for reference.

　　Methods HeLa cells were infected with PCV2, and then analyzed by RNA-seq technology to screen differentially expressed genes (DEGs) related to antiviral response, and further verified by real-time quantitative PCR.

　　Results A total of 15,402 UniGenes were annotated in PCV2-infected HeLa cells, including 387 DEGs (including 267 up-regulated genes and 120 down-regulated genes). GO enrichment and KEGG pathway analysis of biological process DEGs showed that the enriched genes were involved in stress response (97 / 291 DEGs) and immune system processes (80 / 291 DEGs), and the most significant two pathway genes were NOD Differentially expressed genes related to receptor-like receptor signaling pathway (21/170 DEGs) and herpes simplex virus infection pathway (22/170 DEGs).

　　Conclusion After the infection of HeLa cells with PCV2, the expression profile of the cells was changed. The obtained DEGs and their functional annotation information are helpful to understand the antiviral mechanism, and provide a basis for understanding the immune response and antiviral efficacy of PCV2-infected human cells.