动物造模,深Ⅱ度烫伤 z-bio 2022-10-11 909

　　Objective To establish a pig model of deep second-degree scald, and to observe the histopathological changes of skin repaired at different time points.

　　Methods The skin deep second-degree scald wounds of miniature pigs were prepared by using a constant temperature scalding apparatus. On the 1st, 3rd, 7th, 4th, 21st, and 28th day, the macroscopic morphological changes of skin wound healing were observed and the wound healing rate was measured. Samples were taken at 1, 14, and 28 days, and the dynamic pathological changes of skin repaired at different time points were observed by HE, Massom staining and immunohistochemical CD31 staining.

　　Results By measuring the wound healing rate, compared with female pigs, the healing rate of scalded wounds in dimensional pigs was faster. After scald, it was confirmed by different staining methods that the damage had accumulated to the deep layer of the dermis, reaching the standard of deep second-degree scald. On the 14th and 28th day of the subsequent selection, the skin tissue underwent tissue repair to different degrees after injury, respectively. After 28 days of deep second-degree burns, the skin of the miniature pigs had basically healed, but the skin tissue was not yet fully mature. In addition to the relatively mature layers of the epidermis, the dermis has not yet fully formed scar tissue.

　　Conclusion Compared with female pigs, the healing rate of scalded wounds in male pigs is faster.