动物造模 z-bio 2022-11-29 292

　　Objective To study the function of interferon-induced transmembrane protein 3 in African green monkeys, as well as the mechanism and mode of action of its antiviral effect, the IFITM3 gene was cloned from different tissues and organs of African green monkeys, and the expression characteristics of the gene were analyzed. analyze.

　　Methods The full-length cDNA of IFITM3 was cloned from the heart, liver, lung, kidney, brain and other tissues and organs of African green monkeys by RT-PCR. DNAMAN software was used to compare the nucleotide differences of IFITM3 cDNA from different species, and the genetic diversity of AGM IFITM3 was analyzed. Real-time PCR was used to detect the expression of IFITM3 gene in various tissues and organs of African green monkeys. , using pathological and immunohistochemical methods to detect the differences in the expression of IFITM3 protein in different tissues and organs of African green monkeys.

　　Results The expression of IFITM3 was detected in the lymphocytes, skin, heart, brain, liver, kidney, lung and spleen of African green monkeys. The obtained IFITM3 cDNA was about 440bp (442~448bp), which was similar to that of macaques in terms of gene evolution. Sexuality reached 93%. The software predicts that the AGM IFITM3 protein contains five highly conserved transmembrane domains and specificity sites. The results of Real-time PCR showed that the expression of IFITM3 gene in African green monkeys was the highest in the spleen and the lowest in the brain, and the results of immunohistochemistry showed that the IFITM3 protein content was the highest in sebaceous glands, spleen and lymphocytes, while brain tissue, Lung and other protein content is lower.

　　Conclusion Based on the widespread existence of IFITM3 in the tissues and organs of African green monkeys, and its high abundance expression in the spleen and lymph of its important immune organs, it is speculated that IFITM3 plays a role in the establishment of a healthy immune system and strong immune capacity in African green monkeys. It plays an important role and has an important relationship with the immunity and adaptability of African green monkeys to important zoonotic virus infections.