动物造模 z-bio 2022-07-27 227

　　Objective To investigate the changes in the structure of intestinal flora in quail with hyperuricemia, and to provide reference for the study of the pathological mechanism of hyperuricemia.

　　Methods Difak quails were randomly divided into two groups: normal group and model group. The normal group was fed with normal feed, while the model group was fed with high-purine feed. PCR-DGGE method was used to construct the DNA fingerprints of the bacteria in the caecal contents of the two groups of quails. The map was digitized, and the overall differences in the intestinal flora structure of the two groups of quails were compared by cluster analysis.

　　Results On the 28th day of modeling, compared with the normal group, the quails in the model group had significantly higher blood uric acid and changes in the overall structure of the intestinal flora. There were 12 different bands on the DGGE fingerprint, and the sequence identification belonged to the phylum Bacteroidetes, Pseudomonas spp. Bacteria, Proteobacteria, and cecal tissue showed inflammatory changes.

　　Conclusion The structure of intestinal flora in quail with hyperuricemia induced by high purine diet is altered.