动物造模 z-bio 2023-03-03 429

　　OBJECTIVE: To preliminarily investigate the in vivo biological properties of the porous composite HAPw/n-ZnO.

　　Methods: Fifteen tree shrews, male or female, were selected. Each back muscle was implanted with porous composite material HAPw/n-ZnO, Bio-Oss bone meal, ATLANTIK artificial bone, domestic Jinshi Zhiguling artificial bone, and porous composite material HAPw/n -ZnO is the experimental group, and the rest are the control group. The general observation of the animals and the observation of the surgical site were carried out after the operation. 4 animals were randomly selected and sacrificed at each time at 4 weeks, 8 weeks and 12 weeks, and the histopathological observation of the muscle at the implantation site and the alkalescence were carried out. Sex phosphatase activity (ALP) and calcium content were measured.

　　Results: HE staining showed that calcification foci were visible in the interstitium of the experimental HAPw/n-ZnO group and each control group, and inflammatory cells were obviously aggregated in the muscles of the experimental group; Masson staining showed that the surrounding of the implant materials in the experimental group and each control group was green. The difference between the experimental group and the Jinshizhiguling group and the Bio-Oss bone meal group was statistically significant, and the difference between the Jinshizhiguling group and the Bio-Oss bone meal group was better than that of the experimental group, and the There was no significant difference in the ATLANTIK artificial bone group.

　　Conclusion: The porous composite material HAPw/n-ZnO has osteogenic activity but not ectopic bone formation after implantation into the back muscles of tree shrew, and causes inflammatory response.