动物造模,自发性2型糖尿病 z-bio 2023-04-27 450

　　Objective: To investigate the basic metabolic characteristics such as glucose and lipid metabolism, body composition, circadian exercise and metabolism in Chinese hamsters with spontaneous type 2 diabetes mellitus and the expression of related genes in skeletal muscle and liver.

　　Methods: According to the fasting blood glucose (FBG) and postprandial blood glucose (PBG) values of Chinese hamsters, the control group (FBG≤4.5mmol/L and PBG<6.0mmol and="" the="" diabetes="" group="" l="" pbg="">7.0 mmol/L), the body weight, blood sugar, blood lipids, serum insulin content and glucose tolerance of the animals were measured, the body composition, circadian movement and metabolic characteristics of the animals were analyzed, and the related genes glucose transporter 4 (Glut4) and peroxides were detected. Expression of peroxisome proliferative activated receptor-γ (Pparg) in skeletal muscle and liver.

　　RESULTS: Compared with the control group, the Chinese hamster diabetic group had increased blood glucose and blood lipid levels, increased serum insulin content and homeostasis model assessment of insulin resistance (HOMA-IR), decreased body fat percentage, food intake and daytime activity The mRNA and protein expression levels of PPARG in liver and skeletal muscle were significantly increased; the mRNA and protein expression levels of GLUT4 in skeletal muscle were significantly decreased.

　　Conclusion: Chinese hamsters with spontaneous type 2 diabetes belong to the non-obese type 2 diabetes animal model with abnormal glucose and lipid metabolism and can produce insulin resistance. The down-regulation of GLUT4 may be related to abnormal glucose metabolism and insulin resistance in skeletal muscle, while the up-regulated PPARG May be beneficial to the relief of the body's insulin resistance state.