动物造模 z-bio 2022-05-11 253

　　Objective: To use genomic data to screen more effective microsatellite loci of gerbils for genetic quality analysis of inbred lines and closed groups, and to enrich the genetic data of gerbils.

　　Methods: Through the analysis of the whole genome sequencing results of the gerbil, 357 repeating sequences that meet the microsatellite criteria were selected, and corresponding primers were designed and synthesized according to the flanking sequence of each site. The genomic DNA of gerbil was extracted and amplified by PCR. After optimization of primer sequences and PCR conditions and analysis of gel electrophoresis experiments, the successfully amplified loci were verified and optimized by different inbred lines and closed groups of gerbils, and the loci suitable for genetic quality analysis were screened out. .

　　RESULTS: Among the 357 microsatellite loci primers, 135 loci primers successfully amplified PCR products. The analysis results showed that 10 loci could distinguish the inbred line of gerbil cerebral ischemia and diabetes model. In 12 closed colony gerbils, 23 loci showed polymorphism.

　　Conclusion: A total of 135 microsatellite loci of gerbils were successfully screened, and some loci can be used for genetic quality analysis of inbred strains and closed groups of gerbils.