动物造模 z-bio 2022-05-12 256

　　OBJECTIVE: To test the quality and developmental potential of mouse dormant embryos after freezing and thawing, and to provide necessary reference for the production and application of embryo dormancy technology.

　　Methods: The normal incubation period embryos and dormant embryos were frozen by conventional freezing method, and then the in vitro recovery culture experiments and embryo transfer experiments were carried out respectively. Then, the dormant embryos and normal incubation period mouse embryos before and after freezing and thawing were analyzed by double fluorescent staining method. Embryos were counted and the quality changes before and after freezing and thawing were observed.

　　Results: The freezing and thawing recovery rate and development rate of dormant embryos were significantly higher than those of hatching embryos (72.1% vs 50.2%, P<0.01; 94.2% vs 73.9%, P<0.01). The transfer pregnancy rate of dormant embryos Significantly higher than that of hatching embryos (40.8% vs 30.1%, P<0.05). The number of inner cell mass cells in dormant embryos was significantly higher than that in hatching embryos (27.83vs19.53, P<0.05), and the difference in the number of trophoblast cells was not significant. Significant. The number of inner cell clusters and trophoblast cells of dormant embryos after freeze-thaw culture were significantly higher than those of embryos during incubation (25.18vs 14.68, P<0.05; 114.09vs 73.88, P<0.05).

　　Conclusion: The embryo quality and developmental potential in vitro and in vivo of mouse dormant embryos after freezing and thawing are better than those of mouse embryos in normal incubation period.