动物造模,肠道菌群 z-bio 2022-10-13 448

　　OBJECTIVE: To observe the changes in the structure and abundance of gut microbiota in rhesus monkeys of different ages.

　　Methods: Fifty rhesus monkeys were divided into two groups: adult (5-10 years old) and elderly (over 10 years old) groups, including 33 in the adult group and 17 in the elderly group. Fresh rectal feces of animals were collected, and DNA was extracted using Illumina The high-throughput sequencing platform sequenced the bacterial 16S rDNA-V3 region in the samples, and quantitatively analyzed the structure and abundance of intestinal flora.

　　Results: There was no significant difference in the number of optimized sequences obtained between the elderly group and the adult group (P>0.05). α-diversity analysis, Chao1 index (P=0.0174), Simpson index (P=0.0174), Simpson index (P Compared with the adult group, the ACE index (P=0.0121) was lower, and the Shannon index was higher than that of the adult group (P = 0.0132). Compared with the adult group, at the phylum level, the phylum Bacteroidetes was relatively higher in the elderly group than in the adult group. The abundance increased (P=0.0013), while the relative abundances of Firmicutes, Spirochaetes, Verrucomicrobia and Lentisphaerae decreased (P =0.028 P = 0.000 P = 0.048 P = 0.0242). At the family level, the relative abundance of Prevotellaceae increased (P = 0.0001), while the relative abundance of Ruminococcaceae, Clostridiales, Spirochaetaceae, Christensenellaceae decreased (P=0.0039, P=0.0080, P=0.000 P= 0.0021). At the genus level, the relative abundance of unidentified_Prevotellaceae in the elderly group increased (P=0.0001), while the relative abundance of Lactobacillus, Sarcina, and unidentified_Spirochaetaceae decreased (P=0.0114, P=0.0227, P=0.0021). =0.0028). β-diversity analysis showed that the intestinal flora of the elderly group and the adult group were distributed in different regions, and the difference was significant (P=0.003). LEfSe analysis showed that in the adult group, Streptococcus, Buch Buchnera and lactobacillus were statistically significant biomarkers.

　　Conclusion: The structure of rhesus monkey gut microbiota changes with age. The abundance decreases and the diversity increases.